鏈霉親和素磁珠
結(jié)合生物素化蛋白(抗體) 1. 取樣:將鏈親和素磁珠溫和漩渦振蕩20 s 重懸均勻;取100 μL 磁珠懸液到 試管中;磁力分離保留鏈親和素磁珠,用移液器吸去上清液; 備注:需要據(jù)待分離生物素化分子的量及大小,參考產(chǎn)品信息表中數(shù)據(jù),確定磁 珠需要量。 2. 洗滌:將1.0 mL Buffer II 與試管中磁珠混勻;磁力分離,移去上清液; 備注:當(dāng)步驟1 取用磁珠體積大于1.0 mL 時(shí),加入與磁珠體積相同的Buffer II。 3. 再洗滌:重復(fù)“步驟2” 兩次,再洗滌鏈親和素磁珠兩次; 4. 吸附:將1.0 mL 用Buffer II 稀釋生物素化蛋白與洗滌后鏈親和素磁珠混勻 (磁珠濃度 達(dá)1.0 mg/mL),溫和充分振蕩重懸;在四維旋轉(zhuǎn)混合儀上,將混合物室溫混合~30 min; 5. 去除未結(jié)合成分:磁力分離保留磁珠,將上清液轉(zhuǎn)移至新的離心管; 6. 洗滌:按“步驟2”的方法,用緩沖液洗滌結(jié)合蛋白的鏈親和素磁珠3 次以上; 7. 測(cè)量:測(cè)定吸附反應(yīng)前后上清液中蛋白濃度,計(jì)算被磁珠結(jié)合蛋白量; 8. 完成:據(jù)后續(xù)實(shí)驗(yàn)要求,加入Buffer II 或其他合適緩沖液重懸磁珠,備用。 提示1. 冷凍、干燥和離心都造成磁珠團(tuán)聚而無法再分散,失去效用; 2. 磁珠用前需重懸均勻,切忌劇烈震蕩產(chǎn)生氣泡導(dǎo)致蛋白變性; 3. 移液器吸頭和反應(yīng)管需避免因粘附磁珠而造成磁珠損失; 上海濤宇國際貿(mào)易有限公司主營化學(xué)發(fā)光TS磁珠,若有需要,歡迎來電。河南Spherotech磁珠多少錢
22-01-103
熒光交聯(lián)右旋糖苷磁珠-紅
Fluorescent
Crosslinked magnetic dextran nanoparticles-red plain
100 nm 5 mg/ml 5 ml
22-02-103
熒光交聯(lián)右旋糖苷磁珠-紅
Fluorescent Crosslinked
magnetic dextran nanoparticles-red
NH2 100 nm 5 mg/ml 5 ml
63-01-103
熒光羥乙基Bionized納米鐵磁珠-紅
Fluorescent magnetic
bionized nanoferrite crosslinked hydroxyethyl starch particles-red plain
100 nm 10 mg/ml 5 ml
63-02-103
熒光羥乙基Bionized納米鐵磁珠-紅
Fluorescent magnetic
bionized nanoferrite crosslinked hydroxyethyl starch particles-red NH2
100 nm 10 mg/ml 5 ml
63-20-103
熒光羥乙基Bionized納米鐵磁珠-紅
Fluorescent magnetic
bionized nanoferrite crosslinked hydroxyethyl starch particles-red streptavidin 100 nm 5 mg/ml 1 ml
93-01-103
熒光羥乙基Bionized納米鐵磁珠-紅
Fluorescent magnetic
bionized nanoferrite crosslinked dextran particles-red plain
100 nm 10 mg/ml 5 ml
93-02-103
熒光右旋糖苷Bionized納米鐵磁珠-紅
Fluorescent magnetic
bionized nanoferrite crosslinked dextran particles-red NH2
100 nm 10 mg/ml 5 ml
內(nèi)蒙古RNA提取磁珠哪家好上海濤宇國際貿(mào)易有限公司 殼聚糖磁珠,若有需要,歡迎來電垂詢。
山羊抗兔IgG(HL)磁珠
GAR04-10
山羊抗兔IgG(HL)磁性微球
Gt anti-Rb IgG (H&L) Magnetic
Particles, 1% w/v, 4.0-4.5 um, 10 mL
GAR041-10
山羊抗兔IgG(Fc)磁性微球
Gt anti-Rb IgG (Fc) Magnetic Particles, 1% w/v, 4.0-4.5 um, 10 mL
GAR03-10
山羊抗兔IgG(HL)磁性微球 光滑
Gt anti-Rb IgG (H&L) Magnetic Particles, Smooth, 1% w/v, 3.0-3.9 um,
10 mL
GARS4-10
山羊抗兔IgG(HL)磁性微球 光滑
Gt anti-Rb IgG (H&L) Magnetic Particles, Smooth, 1% w/v, 4.0-4.5 um,
10 mL
GAR3-10
山羊抗兔IgG(Fc)磁性微球 光滑
Gt anti-Rb IgG (Fc) Magnetic Particles, Smooth, 1% w/v, 3.0-3.9 um, 10 mL
GAR015-10
山羊抗兔IgG(HL)磁性微球 交聯(lián)
Gt anti-Rb IgG (H&L) Magnetic Particles, Crosslinked, 0.5% w/v,
1.0-2.0 um, 10mLGAR0825-100
山羊抗兔IgG(Fc)聚苯乙烯微球
Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.25% w/v, 0.7-0.9 um, 100 mL
GAR0825-20
山羊抗兔IgG(Fc)聚苯乙烯微球
Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.25% w/v, 0.7-0.9 um, 20 mL
GAR75-5
山羊抗兔IgG(Fc)聚苯乙烯微球
Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.5% w/v, 6.0-8.0 um, 5 mL
RAC-4X 成份:BCP62-2,RFP62-2,RCP32-2,RCP37-2
AVP17-5
卵白素(親和素)熒光微球 粉色
Avidin Coated Fluorescent Particles, Pink, 0.1% w/v, 1.7-2.2 um, 5 mL
AVM015-10
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 0.5% w/v, 1.0-1.4 um, 10mL
AVM4-10
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 1% w/v, 4.0-4.5 um, 10 mL
AVM4-100
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 1% w/v, 4.0-4.5 um, 100 mL
AVM7-10
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 1% w/v, 6.0-8.0 um, 10 mL
AVM7-100
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 1% w/v, 6.0-8.0 um, 100 mL
AVM8-5
卵白素(親和素)磁性微球
Avidin Coated Magnetic Particles, 1% w/v, 8.1-9.9 um, 5 mL
AVMS3-10
卵白素(親和素)磁性微球 光滑
Avidin Coated Magnetic Particles, Smooth, 1% w/v, 3.0-3.9 um, 10 mL
上海濤宇國際貿(mào)易有限公司 主營Spherotech鏈霉親和素編碼磁珠若有需要,歡迎來電垂詢。
MDAG041-10
驢抗山羊IgG(HL)磁性微球
Donkey anti-Gt IgG (H&L), Magnetic
Particles, Crossadsorbed with Ck, GP, Sy, Hms, Hrs, Hu, Ms, Rb, Rat Sr
Protein, 1% w/v, 4.0-4.5 um, 10 mL
PDAG075-5
驢抗山羊IgG(HL)聚苯乙烯微球
Donkey anti-Gt IgG (H&L) Polystyrene Particles, Crossadsorbed with
Ck, GP, Sy, Hms, Hrs, Hu, Ms, Rb, Rat Sr Protein, 0.5% w/v, 6.0-8.0 um, 5mL
GPB10-2
谷胱甘肽 熒光聚苯乙烯微球
白藍(lán)色
Glutathione Fluorescent Polystyrene Particles, PAK Blue, 1E7/mL,
10.0-14.0 um, 2 mL
GP025-5
谷胱甘肽 聚苯乙烯微球
Glutathione Polystyrene Particles, 0.5% w/v, 2.0-2.9 um, 5 mL
GP045-5
谷胱甘肽 聚苯乙烯微球
Glutathione Polystyrene Particles, 0.5% w/v, 4.0-4.9 um, 5 mL
GP075-5
谷胱甘肽 聚苯乙烯微球
Glutathione Polystyrene Particles, 0.5% w/v, 6.0-8.0 um, 5 mL
MHS03-10
羥乙基磁性微球 光滑
Hydroxyethyl Magnetic Particles, Smooth, 2.5% w/v, 3.0-3.9 μm, 10 mL
GAHY08-5
山羊抗人IgG(HL)熒光微球 黃色
Gt anti-Hu IgG (H&L) Fluorescent Particles, Yellow, 0.1% w/v, 0.7-0.9
um, 5 mL
MGAH4-10
山羊抗人IgG(HL)磁性微球
Gt anti-Hu IgG (H&L) Magnetic Particles, 1% w/v, 4.0-4.5 um, 10 mL
MHS100
HandiMag 磁性分離微球
HandiMag Magnetic Separator
上海濤宇國際貿(mào)易有限公司二氧化硅磁珠200nm 磁珠,若有需要,歡迎來電。河北蛋白A抗體磁珠廠家現(xiàn)貨
上海濤宇國際貿(mào)易有限公司 主營Spherotech多重分析熒光編碼SA磁珠若有需要,歡迎來電垂詢。河南Spherotech磁珠多少錢
MSV2-5
鏈霉親和素包裹強(qiáng)磁微球
Streptavidin Coated FerroMagnetic Particles, 1% w/v, 2.0-2.9 um, 5mL
MSV4-5
鏈霉親和素包裹強(qiáng)磁微球
Streptavidin Coated FerroMagnetic Particles, 1% w/v, 4.0-4.5 um, 5 mL
SF055-5
鏈霉親和素包裹熒光微球 黃色
Streptavidin Coated Fluorescent Particles, Yellow, 0.1% w/v, 0.4-0.6 um, 5 mL
SF056-5
鏈霉親和素包裹熒光微球 紅色
Streptavidin Coated Fluorescent Particles, Nile Red, 0.1% w/v, 0.4-0.6 um, 5 mL
CF106-5
鏈霉親和素包裹熒光微球 藍(lán)色
Streptavidin Coated Fluorescent Particles, Blue, 0.1% w/v, 1.0-1.9 um, 5 mL
SF605-5
鏈霉親和素包裹熒光微球 紅色
Streptavidin Coated Fluorescent Particles, Nile Red, 0.1% w/v, 5.0-7.9 um, 5 mL
MSV55-10
鏈霉親和素包裹磁性微球
Streptavidin Coated Magnetic Particles, 0.5% w/v, 0.5-0.69 um, 10 mL
MSV85-10
鏈霉親和素包裹磁性微球
Streptavidin Coated Magnetic Particles, 0.5% w/v, 0.7-0.9 um, 10 mL
MSV015-10
鏈霉親和素包裹磁性微球
Streptavidin Coated Magnetic Particles, 0.5% w/v, 1.0-1.4 um, 10 mL
河南Spherotech磁珠多少錢